Table 2. List of the oligonucleotides used in this study.
Primers’ ID | 5′ → 3′sequence | PCR product length (bp) | Total G + C content (bp) |
---|---|---|---|
OPV-HRM-For | TAGGACTAGCCGCGGTAACTT | 56 | 21 |
OPV-HRM-Rev | ACAAGATAGAAGCGATGGATACTT | ||
CaPV-HRM-For | TCCTGGCATTTTAAGTAATGGT | 100 | 38 |
CaPV-HRM-Rev | GTCAGATATAAACCCGGCAAGTG | ||
PPV-HRM-For | TCGAAGATCTTGTCCAGGAAG | 112 | 57 |
PPV-HRM-Rev | CCGAGAAGATCAACGAGGTC |
The names and sequences of the primers designed to amplify short fragments in the targeted virus genomes and the estimated PCR amplicon size and G + C content are presented.