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. 2017 Feb 20;7:42853. doi: 10.1038/srep42853

Figure 5. DDX6 FL and CTD bind to the condensed chromosomes during M phase.

Figure 5

(a) YFP-DDX6 FL and CTD were associated with mitotic chromosomes. HeLa cells transfected with EYFP, YFP-DDX6 FL, -NTD, or -CTD were synchronized at prometaphase using nocodazole (50 μg/mL) and imaged. Images were acquired with fluorescent microscope (a) and confocal microscope (b), respectively. (c) DDX6 CTD accounts for mitotic chromosome association. Synchronized HeLa cells expressing YFP-DDX6 FL, YFP-DDX6 Rec (Fig. S15), YFP-PKM-DDX6 CTD, and YFP-PKM were imaged with fluorescent microscope. (d) Truncation in DDX6 CTD abolishes chromosome association. HeLa cells expressing YFP-DDX6 CTD-Δ1 and -Δ2 were synchronized and imaged with fluorescent microscope. Fractions represent numbers of cells with displayed phenotypes over total counted cells. (e) Bar graph showing the phenotype statistics. Data was calculated from three biological replicates as shown in (a, c and d). (a–d) YFP signal is shown in green. Mitotic chromosomes were stained with Hoechst. Scale bar = 10 μm. Granule+: granule-positive phenotype. Double+: granule/chromosome double-positive phenotype. Chr.+: chromosome-positive phenotype. (a, c and d) Numbers represent means and standard deviations of the percentage of the displayed phenotypes calculated from 3 replicates.