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. 2017 Feb 20;7:42680. doi: 10.1038/srep42680

Figure 3. Direct post-transcriptional regulation of LIN28B expression through miR-203.

Figure 3

(A) The results of a quantitative RT-PCR analysis of the miR-203 levels in A549 and 95D cells treated with the control agomir, miR-203 agomir, control antagomir or miR-203 antagomir. (B and C) The results of a Western blot analysis of the LIN28B protein levels in A549 and 95D cells treated with the control agomir, miR-203 agomir, control antagomir or miR-203 antagomir. (B) A representative image; Full-length blots/gels are presented in Supplementary Fig. 6. (C) The results of a quantitative analysis. (D) The results of a quantitative RT-PCR analysis of the LIN28B mRNA levels in A549 and 95D cells treated with the control agomir, miR-203 agomir, control antagomir or miR-203 antagomir. (E) Firefly luciferase reporters containing either wild-type (WT) or mutant (Mut) miR-203 binding sites in the LIN28B 3′-UTR were co-transfected into A549 and 95D cells along with control agomir, miR-203 agomir, control antagomir or miR-203 antagomir. The cells were assayed using a luciferase assay kit 24 h post-transfection. The results are presented as the means ± SE of three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001).