Figure 3. Rp9 gene mutation inhibited the proliferation and migration of 661 W cells.

(A) MTT cell proliferation assay was performed at indicated days. Data at each time point were expressed as mean ± SEM based on results obtained from triplicates. **P < 0.01, ***P < 0.001. (B) Rp9 gene mutation down-regulated Ccnd2 expression. 661 W, Rp9-KI and Rp9-KO cell lysate were prepared and used for Western blot analysis. Gapdh was used as an internal loading control. The band intensity was measured with ImageJ software, the fold change was normalized to the level of 661 W group. Data are representative of three independent experiments. **P < 0.01. (C) In vitro scratch assays were performed to evaluate the migration potential of 661 W, Rp9-KI and Rp9-KO cells.