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. 2017 Feb 20;7:43062. doi: 10.1038/srep43062

Figure 5. Rp9 gene mutation significantly affects Fscn2 pre-mRNA splicing.

Figure 5

(A) qRT- PCR analysis of Fscn2 and Bbs2 transcripts in 661 W, Rp9-KI and Rp9-KO cells. Relative expression levels of mRNA were normalized against Gapdh. ***P < 0.001. (B) Genomic structures of Fscn2 and Bbs2 genes and primers used for RT-PCR. (C) Effects of Rp9 gene mutation on the pre-mRNA splicing of Fscn2 intron 3, intron 4 and Bbs2 intron 8, intron 10. RT-PCR was used to detect the splicing products and corresponding pre-mRNA using specific primers as depicted in panel B. (D) Quantification of Fscn2 and Bbs2 splicing efficiency by measuring the ratio of mRNA to pre-mRNA using ImageJ from three independent experiments. *P < 0.05.