Fig. 3.

Camera Response Function calibration experiment. In a is reported one of the sets of brightfield images of fixed eukaryotic cells used during this preliminary test. The same field of view was captured at different exposure times (between 1 and 4 ms). The third degree polynomial function representing the CRF (blue line) is plotted together with the data on which it was fitted (red, green and yellow dots in b). The different colors identify the pixels belonging to each image pair