Fig. 2.

Completed 3D-printed unibody CL immunoarray device. (A) Upstream reagent reservoirs are separated by empty air chambers, followed by the 3D mixing network in the center and a detection chamber downstream housing the antibody array. Blue dye solution indicates the fluidic path of the device. (B) A modified 3D-printed device to demonstrate the mixing ability of the passive 3D mixing network. The mixer contains with 96 0.8 mm× 0.8 mm × 0.8 mm 90° turns in a 2 cm × 3.5 cm space. Yellow and blue dyes were pumped at 50 µL min⁻¹ into the mixer network with solution mixing by the third turn (red arrow) demonstrating excellent mixing efficiency of this passive mixer. There was a clear separation of two dyes before the dyes entered the mixer (red circle).