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. Author manuscript; available in PMC: 2017 Apr 28.
Published in final edited form as: J Med Chem. 2016 Apr 12;59(8):3840–3853. doi: 10.1021/acs.jmedchem.6b00003

Figure 2.

Figure 2

TDP1 inhibition assay gel. The concentrations of positive controls 5 and 61 and test compounds were 1.4, 4.1, 12.3, 37, and 111 μM (left to right). The 5′-labeled N14Y TDP1 DNA substrate corresponds to a 3′-phosphotyrosyl 14-mer oligonucleotide and the 5′-labeled N14P DNA product corresponds to a 3′-phosphate 14-mer oligonucleotide (see Experimental Section). Gel-based assays are usually run in two independent experiments.