Figure 3.
TDP2 inhibition assay gel. The concentrations of positive control 6 were 0.006, 0.017, 0.05, 0.15, and 0.46 μM and the concentrations of 61 and the test compounds were 0.46, 1.4, 4.1, 12.3, 37, 111 μM (left to right). The 3′-labeled Y19 TDP2 DNA substrate corresponds to a 5′-phosphotyrosyl 19-mer oligonucleotide and the 3′-labeled p19 TDP2 DNA product corresponds to a 5′-phosphate 19-mer oligonucleotide (see Experimental Section).