Fig. 2.

Application of the column agglutination technique for the identification of anti-CD59. RBCs of the CD59-deficient patient of an unselected donor or antibody screening cells were incubated with antibodies at 37°C as indicated. Either MoAbs or plasma of the CD59-deficient patient were used or plasma of the CD59-deficient patient to which human alloantibodies had been added. After centrifugation of the gel cards, agglutination indicated reactivity.