Fig. 2. BF-TEM tomography for nanoparticles coated by PEG 1 kDa + PNIPAM 1.2 kDa and stained with CuSO4. The tilt series was reconstructed with the SIRT algorithm (Astra Toolbox, see ESI†). Slices through the reconstruction show the absence of a shell (C and F), the presence of a complete shell (A and D) and a half shell (B and E) around the particle (indicated by white arrows) dependent on the position of the slice. In figures D, E and F the segmentation is shown on top of the orthoslices. The scale bar applies to all images.
