Figure 8.

CLs decrease antigen degradation in BMDCs.

Notes: After overnight treatment of different doses of CLs or ALs, BMDCs were incubated for 15 min at 37°C with 10 μg/mL DQ-OVA. The green fluorescence of DQ-OVA was monitored by flow cytometric analysis. BMDCs incubated with DQ-OVA at 4°C were used as control group. The data were analyzed by one-way ANOVA, followed by a multiple comparison post-test. ***P<0.001. Data represent the mean ± SD from three repeated experiments with n =2–4.

Abbreviations: ALs, anionic liposomes; ANOVA, analysis of variance; BMDCs, bone marrow-derived dendritic cells; CLs, cationic liposomes; DC-Chol, 3β-[N-(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol; DOTAP, 1,2-dioleoyl-3-trimethylammonium-propane; PBS, phosphate-buffered saline; OVA, ovalbumin.