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. 2017 Feb 16;12:1317–1328. doi: 10.2147/IJN.S123930

Figure 2.

Figure 2

hBMMSC adhesion and cytoskeleton were evaluated on the four surfaces after 3 days.

Notes: SEM images of hBMMSCs at magnifications of ×100 and ×1,000. Representative CLSM images of cells stained with DAPI to show the nuclei (blue) and FITC to show the actin filaments (green). CON represents control titanium surface; HF1, HF2, and HF3 represent cells, respectively, on the surfaces treated with 1% HF etched for 3 min, 0.5% HF etched for 12 min, and 1.5% HF etched for 12 min.

Abbreviations: hBMMSCs, human bone marrow-derived mesenchymal stem cells; SEM, scanning electron microscopy; CLSM, confocal laser scanning microscopy; DAPI, 4′,6′-diamidino-2-phenylindole; FITC, fluorescein isothiocyanate; HF, hydrofluoric acid.