Figure 5.

Pretreatment with P2Y₁₂R inhibitor MRS2395 and p38MAPK inhibitor SB-203580 suppresses the expression of IL-1β and IL-6, but not TNF-α, in the L4–L6 spinal cord from bone cancer rats using the Western blot.

Notes: The production of IL-1β (A), IL-6 (B), or TNF-α (C) was increased in the cancer + DMSO group than in the sham groups. The increased expression of IL-1β and IL-6 was suppressed by intrathecal injection of MRS2395 (n=4) and SB-203580 (n=4) except TNF-α. *P<0.05 vs sham group and ^#P<0.05 vs cancer + DMSO group.

Abbreviations: IL-1β, interleukin-1β; IL-6, interleukin-6; P2Y₁₂R, P2Y₁₂ receptor; p38MAPK, p38 mitogen-activated protein kinase; TNF-α, tumor necrosis factor-α; DMSO, dimethylsulfoxide; MRS, 2,2-Dimethyl-propionic acid 3-(2-chloro-6-methylaminopurin-9-yl)-2-(2,2-dimethyl-propionyloxymethyl)-propyl ester; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.