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. 2017 Feb 16;10:935–944. doi: 10.2147/OTT.S123398

Figure 4.

Figure 4

Viability repression, apoptosis and autophagy induced by PD were inhibited by 3-MA in RPMI 8226 cells. Cells were treated with 200 μmol/L PD alone or in combination with 10 mmol/L 3-MA for 24 h. (A) Cell viability was assessed by CCK-8 assay. (B) The apoptosis rates of RPMI 8226 cells were determined by flow cytometry. (C and D) Western blot analysis of Bcl-2, Bax, caspase-3, cleaved caspase-3, caspase-9 and cleaved caspase-9. β-actin was used as a control. (E) Quantification analysis of cleaved caspase-3 and cleaved caspase-9 by densitometry. (F) Western blot was used to analyze the expressions of Beclin 1, Atg5, LC3I and LC3II. β-actin was used as a control. (G) Quantification analysis of Beclin 1, Atg5, LC3I and LC3II. (H) The ratio of LC3II/LC3I. Data are presented as mean ± SD. *P<0.05, **P<0.01, ***P<0.001, compared to control.

Abbreviations: SD, standard deviation; PD, polydatin; NC, negative control; CCK-8, cell counting kit8.