Extended Data Figure 8. Ptpn11^E76K/+ MSPCs recruit monocytes, but not macrophages, to the vicinity.

a, Bone sections (one section per femur or tibia) prepared from Ptpn11^E76K/+Prx1-Cre⁺ (n = 4 mice) and Ptpn11^+/+Prx1-Cre⁺ mice (n = 3 mice) at 6–7 months old were immunostained with the indicated antibodies and counterstained with DAPI. b–d, Bone sections (one section per femur or tibia) prepared from Ptpn11^E76K/+Nestin-Cre⁺ and Ptpn11^+/+Nestin-Cre⁺ mice at 7–10 months old were immunostained with the indicated antibodies and counterstained with DAPI (n = 5 mice per group) (b). The distance of HSCs (Lin^–CD48^–CD41^–CD150⁺) from closest CD31⁺CD144⁺ endothelial cells was determined (n = 8 mice per group) (c). The spatial relationship between HSCs (Lin⁻CD41⁻CD150⁺) and megakaryocytes (CD41⁺) was examined. HSCs within <8 µm of megakaryocytes were considered as close to megakaryocytes (n = 5 mice per group) (d). Representative images are shown in all panels.