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. 2017 Feb 20;474(5):699–713. doi: 10.1042/BCJ20160941

Figure 2. In vitro inhibition of CDK16 activity.

Figure 2.

E. coli-purified CDK16 (125 ng) was incubated with rebastinib (A), dabrafenib (B) or indirubin E804 (C) for 5 min followed by the addition of COS1-purified FLAG-cyclin Y (125 ng) and assayed for kinase activity using PCTAIRE-tide as described in ‘Materials and Methods’. Curves were fitted using non-linear regression to the log (inhibitor) vs. response (variable slope) equation using the Graphpad Prism software. Results are expressed as mean ± SD and are shown as percentage activity relative to non-treated kinase, and represent the average of three independent experiments performed using the same protein preparations.