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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: Arch Biochem Biophys. 2017 Jan 31;616:47–58. doi: 10.1016/j.abb.2017.01.011

Figure 7.

Figure 7

Activating and inhibitory effects of divalent metal ions on steady-state catalysis by NPADH. A) The rate of NADH production by 0.1 μM NPADH reacting with 10 μM PAL and 50 μM NAD+ in 50 mM POPSO buffer at pH 7 in the presence (□) or absence (■) of 50 μM NADH as a function of (A) Mg2+ and (B) Mn2+. Best fits through the data according to equation 4 are represented by the solid curves passing through the plotted data.