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Specific detection of admixed alloantibodies in the presence of anti-Sc5. All RBC samples are agglutinated by mixtures of anti-Sc5 with anti-Jka and with anti-S (top panels). Hence, the admixed antibodies with common specificities are masked. Adding soluble recombinant Scianna protein inhibits the anti-Sc5 component of the serum. The clinically relevant alloantibodies to Jka and to S can be identified easily (bottom panels).
