Fig. 2.

Mixture of Scianna and JMH proteins. The three RBC samples for antibody screening are agglutinated by anti-Sc1 and anti-JMH (top panels), which can mask an anti-Fy^a. Adding a mixture of soluble recombinant Scianna and JMH proteins reveals the clinically relevant anti-Fy^a (bottom panels). It is detected in a single step with the use of one high-yield cocktail without prior knowledge of the involved antibodies to high-prevalence antigens in the Scianna and JMH blood group systems.