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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Int J Cancer. 2017 Feb 6;140(7):1597–1608. doi: 10.1002/ijc.30575

Figure 5. VAR2CSA specifically binds to ofCS on Burkitt lymphoma cell lines.

Figure 5

(A) Specificity of rVAR2 binding to the EW36 cell line was tested in flow cytometry by co-incubating 200 nM rVAR2 with a 2-fold dilution of CSA and HS. Percentage of binding towards the control is shown. Data shown as mean (two or three independent experiments) ± SEM. (B) Same as (A) for the JLP119 cell line. (C) Same as (A) for the KK124 cell line. (D) Same as (A) for the SHO cell line. (E) Specificity of rVAR2 binding to four different BL cell lines. The graph shows percentage of binding of rVAR2 to the different cell lines. A control with a domain of the protein not involved in binding to ofCS is shown (rDBL4). Additionally, specificity controls after ChAC treatment and with co-incubation of rVAR2 with 200 ug/ml CSA and HS are also shown.