Table 1. HPLC analysis of the CS structure in selected BL cell lines.
Disaccharide analysis by AMAC labelling and HPLC of CS isolated from the cells. The disaccharides were identified by comparison to commercially available standards. The table shows the CS concentration as well as the percentage 4-O- or 6-O-sulfation for the different cell lines.
| CS [pmol/million cells] | % 4-O-sulfation | % 6-O-sulfation | |
|---|---|---|---|
| Cell line | |||
| EW36 | 160.0 | 35.0 | 6.4 |
|
| |||
| JLP119 | 312.7 | 43.9 | 5.8 |
|
| |||
| KK124 | 267.3 | 59.2 | 4.5 |
|
| |||
| SHO | 152.4 | 24.8 | 10.2 |