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. Author manuscript; available in PMC: 2018 Feb 20.

Published in final edited form as: Chem Res Toxicol. 2017 Jan 3;30(2):635–641. doi: 10.1021/acs.chemrestox.6b00374

Recombinant PKM2 was modified in vitro with increasing concentrations of A) HNE or B) ONE for 1 h at 37°C. PKM2 activity was assessed indirectly by the lactate dehydrogenase-coupled enzyme assay. Data represent an n=3 ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 by ANOVA.

Recombinant PKM2 was modified in vitro with increasing concentrations of A) HNE or B) ONE for 1 h at 37°C. PKM2 activity was assessed indirectly by the lactate dehydrogenase-coupled enzyme assay. Data represent an n=3 ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 by ANOVA.