Figure 6. Oligomer formation inhibits kinase activity in cellular PKM2.
RKO cells were treated with increasing concentrations of HNE or ONE for 1 h, lysed, reduced with NaBH4, and subjected to in vitro PKM2 kinase assays. Data represent the mean ± SD (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001 by ANOVA.