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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Matrix Biol. 2016 Aug 20;60-61:110–123. doi: 10.1016/j.matbio.2016.08.003

Figure 3.

Figure 3

A three-dimensional reconstruction of a hydrated fibrin network. The clot was formed in human citrated platelet-poor plasma at room temperature for 30 minutes after addition of calcium chloride (20 mM) and thrombin (0.5 U/ml). To visualize fibrin network structure using fluorescent confocal microscopy, Alexa-Fluor 488-labeled human fibrinogen was added to the plasma sample before clotting at a final concentration of 0.04 mg/ml. The network was imaged using a Zeiss LSM710 laser scanning confocal microscope to generate z-stack images spanning 100 μm of sample thickness, with a distance of 0.5 μm between slices and 1024×1024 pixel resolution for each slice.