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. Author manuscript; available in PMC: 2017 Feb 21.
Published in final edited form as: Mol Pharm. 2016 Feb 22;13(3):1176–1184. doi: 10.1021/acs.molpharmaceut.6b00078

Figure 2.

Figure 2

Flow cytometry analysis using anti-CD44-BV421 antibody labeling to determine the CD44 receptor expression levels in CD44+ HeLa cells, CD44− HeLa cells, and CD44− NIH 3T3 fibroblasts. The CD44 receptor expression levels were determined by plating 75,000 cells per well in 24-well plates and incubating for 24 h before the experiment. Cells were then stained with CD44 antibody (mouse anti-human-CD44 antibody labeled with BV421) at a concentration of 1μg/mL in DMEM media for 30 min. After incubation, the spent media was removed and the cells were washed 3 times with PBS before trypsinization. The cells were then collected and analyzed using a BD FACS Aria III flow cytometer. Data points represent group mean ± SD (n = 6; ***P < 0.005; ANOVA).