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Confocal microscopy z-stack images of HeLa cells treated with HA-Ad:CD-PEI +:pDNA complexes at N/P = 20 as a function of incubation time (indicated time, h). Green: pDNA-FITC cargo in HA-Ad:CD-PEI+:pDNA complexes; Blue: plasma membrane labeled with Alexa680-WGA; Red: lysosomes labeled with Lysotracker DND-99.
