Fig. 4.

The absorption spectras of metHb (5 µM) and pDNA (25 µg/ml) were recorded every 30 min for 12 h at 37 °C. A) The absorption spectra of HbA was not altered in presence of pDNA, however, gradual decrease in peak intensity of HbA (without pDNA) was observed (A, inset). B) The peak intensity of HbF, in presence of pDNA was increased over the time. HbF alone did not showed any change in absorbance during an incubation period (B, inset).