Figure 3.

Quantification of chromatin-bound Ino80 in wild-type, Arp5 KO, and Arp8 KO cells. (A) The chromatin fractions prepared from the indicated cells were immunoblotted with an anti-Ino80 antibody (top panel) and an anti-H3 antibody (lower panel), which was used as an internal control. (B) The intensity of the chromatin-bound Ino80 band was normalized with respect to that of H3 band in each case, and the relative amount of chromatin-bound Ino80 in each cell line was normalized with respect to that in the wild-type cells. Student's t-test was used to determine the P-value. ^*P < 0.05.