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. Author manuscript; available in PMC: 2017 Feb 21.
Published in final edited form as: Cell Rep. 2016 Sep 20;16(12):3260–3272. doi: 10.1016/j.celrep.2016.07.052

Figure 3. α-Galcer treatment results in an early influx of neutrophils that are necessary for iNKT cell migration out of the vasculature.

Figure 3

A. Flow cytometry was utilized to determine dynamics of neutrophil influx in the lung and spleen post i.v. α-Galcer treatment. Graphs represent frequency of neutrophils as a percentage of CD45+ cells. B. Extravasation of neutrophils post α-Galcer treatment as in (1E). C. Quantification of neutrophil percentages in total lung homogenate of CD1dKO mice and wild-type littermates. D. Representative IVM images after 24 hours of α-Galcer treatment in istoype control and neutrophil depleted mice. Cell types are labeled respectively. White dotted line defines the shadow left by CXC6GFP+ cells on TRITC-dextran channel. E. Quantification of IVM images in (D). F. iNKT cell localization measured using anti-CD45 infusion in neutrophil depleted and isotype treated mice after i.v. α-Galcer treatment. G. iNKT cell activation status measured using CD69 expression in neutrophil depleted or isotype treated mice after i.v. α-Galcer treatment. Error bars represent standard error of mean. ‘*’ represents P < 0.05. N = 3–5 animals per group.