Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Feb 21;5:15. doi: 10.3389/fped.2017.00015

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 dela Peña-Ponce, Rodriguez-Nieves, Bernhardt, Tuck, Choudhary, Mengual, Mollan, Hudgens, Peter-Wohl and De Paris.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Amnis^®-ImageStreamX analysis of STAT6 activation. The images are representative examples of whole blood samples that were untreated (left panels) or stimulated with IL-4 for 15 min (right panels) and then stained and analyzed for total (unphosphorylated) STAT6 and activated pSTAT6. Each image row shows a single cell labeled with CD3 (blue), CD4 (pink), unphosphorylated STAT 6 (yellow), or phosphorylated STAT6 (pSTAT6; green). The first two rows represent examples of cord blood (CB) stained with STAT 6 or pSTAT6; followed by an 8-month old infant blood sample, and one adult donor blood. Channel 1 (“Ch1”) shows the brightfield image of the cell in relation to the 10-μm size marker.