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. 2017 Feb 21;5:15. doi: 10.3389/fped.2017.00015

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Copyright © 2017 dela Peña-Ponce, Rodriguez-Nieves, Bernhardt, Tuck, Choudhary, Mengual, Mollan, Hudgens, Peter-Wohl and De Paris.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IFN-γ-induced JAK/STAT signaling. (A) The percentages of pJAK2⁺CD4⁺ T cells in cord blood (CB, n = 10), 4- to 6-month-old (n = 3), and 10- to 14-month-old (n = 10) infants. Median frequencies of adult (n = 12) pJAK2⁺CD4⁺ T cells with the 25th and 75th percentile are indicated by the gray shaded area. (B) Representative pJAK2 histograms from a CB sample and an adult blood donor. Note that baseline pJAK2 levels are similar in CB and adult blood, and in IFN-γ stimulated samples, the FMO controls also do not differ between CB and adult blood. In contrast, there is an induction of pJAK2 in adult, but notCB, CD4⁺ T cells after IFN-γ stimulation. (C) The association between pJAK2⁺ and STAT1⁺ CD4⁺ T cells in relation to age through 14 months. Note that the analysis only included infant samples that had sufficient volume to measure both parameters. (D) The associations between pSTAT1⁺CD4⁺ T cells and age through 14 months. Correlations were determined by Spearman rank test. (E) Representative examples of CB, 6-month-old infant and adult blood samples analyzed by Amnis^®-ImageStreamX for the nuclear localization of STAT1 and pSTAT1 in unstimulated samples (left panels) or after IFN-γ stimulation (right panels). The legend is as described in Figure 3.