FIGURE 1.

Overview of the multiplex ligation-dependent probe amplification (MLPA) method. MLPA is comprised of three main stages: designing the probes, performing the multiplex MLPA assay and data collection and analysis. The three stages are described in the detail in the main text. TSS, target-specific sequence; 5′-phos: phosphorylation at the 5′ end of the oligonucleotide. Note that Arabidopsis, as a self-pollinating plant, typically carries pairs of identical alleles. For simplicity, single alleles are depicted.