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. 2017 Feb 21;11:84. doi: 10.3389/fnins.2017.00084

Figure 1.

Figure 1

Effects of long-term ethanol consumption on NMDAR subunit expression in wild-type and F639A mice. Tissue was collected from mice used in a drinking study reported in den Hartog et al. (2013). In that study, wild-type and F639A C57/S129 male mice were given 24 h access to increasing concentrations of ethanol (3–15%; plus 0.2% saccharine) followed by 5 weeks of 20% ethanol (plus 0.2% saccharin) and 5 weeks of 40% ethanol (plus 0.2% saccharin). Twenty-four hours after the last drinking session, tissue punches from orbitofrontal cortex (OFC), medial prefrontal cortex (mPFC), dorsal striatum (DS), nucleus accumbens (Nacc), hippocampus (HC), and basolateral amygdala (BLA) were collected and analyzed for GluN1 (A), GluN2A (B), and GluN2B (C) subunit expression. Mean (± SEM; N) optical density values for protein bands are shown as percent of wild-type controls run the in same blot. Symbol: significant effect of genotype on protein expression of GluN2A (*p < 0.05). Inset shows representative example of western blot showing NMDA subunit expression in wild-type (WT) and F639A (Het) mice.