Figure 7.

NMDAR subunit expression in mice tested for locomotor sensitization to ethanol. One day following the last ethanol injection, tissue was isolated from the medial prefrontal cortex (mPFC), nucleus accumbens (NAcc), and basolateral amygdala (BLA) of wild-type and F639A mice and was analyzed for GluN1 (A), GluN2A (B), and GluN2B (C) subunit expression. Mean (±SEM) optical density values for protein bands are shown as percent of corresponding saline controls (A–C) or wild-type controls (D–F) run the in same blot. Symbol: value significantly different from corresponding control (^*p < 0.05; ^**p < 0.01). Inset shows representative example of western blot showing NMDA subunit expression in saline (S) and ethanol (E) treated wild-type (WT) and F639A (Het) mice.