Figure 4.

Diagram illustrating the relative density of labeling for neuropilin 1 (NP1) mRNA in dorsal root ganglion neurons at different survival times (expressed in days = d) after dorsal root transection (A) or sciatic nerve transection (=SNT) or sciatic nerve crush (=Cr) (B). Each dot represents an analyzed neuron and a horizontal bar indicates the median density at each survival time. The asterisks refer to results obtained with one-way ANOVA Kruskal–Wallis statistics (Dunn’s Multiple Comparison Test; ***a difference between controls and the experimental group that is significant according to the test; P < 0.001). There was a transient upregulation in the expression of NP1 mRNA after dorsal root transection [panel (A) and after sciatic nerve injury panel (B)]. (C) The diameter (expressed in microns) of the examined dorsal root ganglion neurons (DRG) has been plotted against the labeling density for NP1 mRNA in control rat DRG and at different survival times after dorsal root transection. Each examined neuron represents a dot in the diagram. The observed upregulation in NP1 after dorsal root transection appeared to affect neurons in all size classes.