Fig. 2. The decreased expression of inflammatory cytokines after treatment of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) inhibitor-pretreated sebocytes with insulin-like growth factor (IGF)-1. (A) The treatment of cultured sebocytes with 10−7 M IGF-1 showed an increase in the gene expression of NF-κB. The treatment of 100 nM NF-κB inhibitor-pretreated sebocytes with 10−7 M IGF-1 showed a significant decrease in the gene expression of NF-κB, interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α. *p<0.05. (B) The treatment of cultured sebocytes with 10−5 M IGF-1 showed an increase in the gene expression of NF-κB. The treatment of 100 nM NF-κB inhibitor-pretreated sebocytes with 10−5 M IGF-1 showed a significant decrease in the gene expression of NF-κB, IL-1β, IL-6, IL-8, and TNF-α. *p<0.05.
