Fig. 3. (A) Histological features of atopic dermatitis (AD)-like skin lesions after treatment with 1% eupatilin. 1% eupatilin cream significantly reduced inflammatory cell infiltration (p<0.05) (H&E, ×200) and also significantly reduced mast cell infiltration in the dermis, compared to the vehicle (p<0.05) (toluidine blue, ×200). (B) The epidermal thickness in five randomly chosen visual fields at ×200 magnification (H&E, ×200). (C) The number of mast cells in five randomly chosen visual fields at ×400 magnification (toluidine blue, ×400). Non-treated (NT), DNCB+vehicle (VH), DNCB+1% eupatilin cream, DNCB+0.03% tacrolimus ointment at day 29 following the first challenge. Data are presented as mean±standard error of the mean (n=5). *p<0.05, compared to the vehicle group.
