Figure 6.

Tracking head rotation and location of freely moving animals. A, The head rotation (top), x (middle), and y (bottom) coordinates of animal position were simultaneously tracked. Four time points corresponding to the four frames (right) are shown, where the animals head direction, and position in the box change from moment to moment. B, The animal’s position over 3 min was tracked and a heat map of the preferred location was created; red, more time and blue, less time. C, The location of two animals in the same enclosure can be distinctly tracked, including each animals head rotation, and position. Pixy tracking is shown by the boxes around the animal’s head.