Figure 2.
TPC1 Maintains Late Endosome and Lysosomal Morphology
(A–E) Representative LAMP1 staining (white) in fibroblasts transfected with scrambled (Scr) siRNA (A) or with two independent siRNAs targeting TPC1 (B and C) or TPC2 (D and E) for 48 hr. Nuclei were stained using DAPI (blue). Zoomed images are displayed in the right panels. Scale bars, 10 μm.
(F) Summary data quantifying LAMP1 intensity as a percentage of scrambled control (± SEM). Data are from 57–496 cells from 3–11 independent knockdowns.
(G–K) Representative electron micrographs of the endo-lysosomal system in fibroblasts transfected with Scr siRNA (G) or with siRNAs targeting TPC1 (H–I) or TPC2 (J–K). Scale bar, 500 nm.
(L) Summary data quantifying clustering of endo-lysosomes expressed as a percentage of the area occupied relative to non-nuclear cytoplasm. Data are from ten cells under each condition and normalized relative to Scr siRNA (± SEM).