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. 2016 Oct 3;36(7):885–898. doi: 10.1038/onc.2016.257

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Copyright © 2017 The Author(s)

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PFDN1 overexpression promotes EMT and increases the motility of lung cancer cells. (a and b) The effect of PFDN1 overexpression on EMT was examined by immunoblotting of E-cadherin and N-cadherin (a) and by changes in cell morphology (b). (c and d) The effect of PFDN1 overexpression on cell migration was examined by a Transwell assay (c) and wound-healing assay (d). (e) The cell invasion ability of PFDN1-overexpressed A549 cells was examined by a matrigel-coated transwell assay. (f) The effect of PFDN1 overexpression on anchorage-independent cell growth was evaluated by a colony-formation assay in soft agar.