Figure 2.

CITK Has a Phylogenetically Conserved Role in Maintenance of Genome Integrity

(A) DAPI-stained metaphase spreads from control and CITK-depleted ONS-76 cells. An acentric isochromatid fragment is indicated in the inset (arrow).

(B) Frequencies of CABs in mock-treated or CITK-depleted ONS-76 metaphases.

(C) Drosophila brain squashes from wild-type (wt) dck¹ and dck² mutant larvae stained with anti-γH2Av antibodies and DAPI.

(D) Frequencies of negative (0–5 γH2Av foci/cell), moderately positive (6–15 foci/cell), or strongly positive (>15 foci/cell) cells in the samples shown in (C).

(E) Diploid DAPI-stained metaphase spreads from control (wt) and mutant (dck¹ and dck²) Drosophila larval brains showing broken chromosomes (arrows and stars).

(F) Frequencies of chromosome aberrations (CABs) in untreated (NT) or irradiated (IR) (4Gy of X-rays) diploid larval brain cells from wild-type or dck mutant larvae.

The scale bars in (A) and (C) represent 5 μm. Two tails unpaired Student’s t test was used for the statistical analysis of these experiments (n = 3–4 per group). Graphs show mean ± SEM. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.