Figure 6.

Apoptosis of CitK-Null Neural Progenitors Is TP53 Dependent

(A) Frozen sections of cerebella from P6 mice of the indicated genotypes stained with TUNEL assay (apoptotic cells, red) and DAPI. Quantification of apoptotic cells in the indicated genotypes is shown. The scale bars represent 50 μm.

(B) Distribution of TUNEL-positive cells in coronal sections of E14.5 embryonic brains; CX, neocortex; GE, ganglionic eminence.

(C) Examples of cortical plate cells in developing neocortices of mice of the indicated genotypes, stained with Nissl and DAPI. The scale bars represent 25 μm.

(D) Flow-cytometric analysis of DNA content in postnatal cortices (P14) from mice of the indicated genotypes.

(E) DAPI-stained ventricular regions of E14.5 cortices from embryos of the indicated genotypes. Note the presence of tripolar metaphases (blue arrows) and anaphases (red arrows); bipolar metaphases and anaphases are indicated by yellow and green arrows, respectively. The scale bars represent 10 μm.

(F) Quantification of normal and aberrant mitoses in the samples shown in (E).

Two tails unpaired Student’s t test was used for the statistical analysis of these experiments (n = 3 mice per group). Graphs show mean ± SEM. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.