Figure 2.

Identification of Bone Vessel Subtypes in the BM Compartment with Arterial and Sinusoidal Vessel Markers

(A) Maximum intensity projection of tile scans showing interconnecting network of Emcn⁺ arterial vessels and sinusoidal capillaries.

(B) Maximum intensity projection of tile scans showing CD31^hi (red) GFP⁺ (green) arterioles and CD31^low sinusoids of Efnb2-GFP mouse calvarium.

(C) Emcn^low arteriole (arrowhead) connects to Emcn^hi sinusoidal capillaries (arrows).

(D) Both arterioles (arrowhead) and sinusoidal capillaries (arrows) are GFP⁺ (green) in Flk1-GFP mice.

(F and G) CD31^hi (red) Efnb2-GFP⁺ (green) arterioles (arrowheads) connect to BM microdomains formed by interconnecting CD31^low sinusoidal capillaries (arrows).

(E and H) SHG signals (blue) indicate bone tissue surrounding the BM cavities. Note that one single plane for SHG is shown. Due to the curved shape of the skull SHG planes were slightly tilted.