Figure 2. Effect of MLE on the anchorage-independent colony formation of HCT116 human colon cancer cells and H1299 human lung cancer cells. HCT116 (A) and H1299 (B) cells on soft agar were incubated with different concentrations of MLE (0 or 700 µg/ml) for 21 days. The number (in HCT116 and H1299 cells) and size (in H1299 cells) of colonies stained with crystal violet were determined under phase contrast time-lapse microscopy (X100), and representative colonies were present. Statistically significant differences between untreated control and MLE-treated cells were indicated with asterisks (*p<0.05, ***p<0.001, by two-tailed student t-test).