Figure 3. Effect of MLE on apoptosis in HCT116 human colon cancer cells and H1299 human lung cancer cells. DAPI staining intensity (A) of HCT116 and H1299 cells incubated with 0, 175, and 700 µg/ml of MLE for 48 h was estimated, and representative nuclear morphology of H1299 cells was shown on the right panel. Statistically significant differences among groups were indicated with different letters (a-c, p<0.05 by Duncan test). Cell cycle distribution (B) of HCT116 and H1299 cells treated with 0 or 700 µg/ml concentration of MLE was analyzed, and the cell population ( %) at sub-G1 (M4) phase was shown. Statistically significant differences between untreated control and MLE-treated cells were indicated with asterisks (*p<0.05 by two-tailed student t-test).