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. 2017 Feb 21;7:42882. doi: 10.1038/srep42882

Figure 3. Determination of a cis-element in Nfil3 IRES.

Figure 3

(a) Schematic diagram of Nfil3 5′-UTR deletion constructs. (b) MC3T3-E1 cells were transfected with the control and deletion constructs and incubated for 24 hours. Cells were harvested at the indicated times, and cell lysates were subjected to a luciferase assay. The ratio of empty vector (pRF) was set to 1.0. Error bars represent mean ± SEM (n = 3), ****P < 0.0001, ns P ≥ 0.05. (c) In vitro transcribed reporter mRNAs containing the Nfil3 5′-UTR were transfected into MC3T3-E1 cells and incubated for 12 hours. Cells were harvested at the indicated times, and cell lysates were subjected to a luciferase assay. The ratio of full-length of Nfil3 (RF-Nfil3) set to 1.0. Error bars represent mean ± SEM (n = 4), **P < 0.01, ns P ≥ 0.05.