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. 2017 Feb 21;7:42882. doi: 10.1038/srep42882

Figure 8. Intracellular calcium level is regulated by Nfil3.

Figure 8

(a) siRNA against endogenous Nfil3 (si_Nfil3), Cacna1c (si_Cacna1c) or control siRNA (si_Con) were transiently transfected into MC3T3-E1 cells. Transfected cells were harvested at 48 hours after transfection and subjected to immunoblotting or quantitative real-time PCR. Nfil3 and Cacna1c mRNA were quantified specific primers and normalized to Gapdh mRNA. The si_Con transfected Nfil3 and Cacna1c mRNA level were set to 1.0. To statistically analyze the each mRNA level, two-tailed unpaired Student’s t-tests was performed. Error bars represent mean ± SEM (n = 3), ***P < 0.001, **P < 0.01. si_Cacna1c was used as a positive control for this assay. Full-length blots are presented in Figure S13 and band of interest is indicated by a red box. (b) MC3T3-E1 cells prepared in panel (a) were subjected to intracellular calcium measurement. The intracellular calcium level in si_Con transfected cells was set to 1.0. Error bars represent mean ± SEM (n = 3), ***P < 0.001, *P < 0.05.