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. 2017 Feb 21;7:42849. doi: 10.1038/srep42849

Figure 6. SDS-PAGE analysis comparing performance of SAVSBPM18-L-ABD(KC) matrix with SAVSBPM18 Affi-gel for purification of biotinylated BSA.

Figure 6

1-ml column containing approximately 1 mg of SAVSBPM18 or its fusion was used for each matrix. 1 mg of biotinylated BSA was loaded to each column. Lanes 1–6 are samples from SAVSBPM18-L-ABD(KC) column. Lanes 7–12 are samples from SAVSBPM18 Affi-gel column. M, molecular weight marker; BM, boiled matrix [2 μl for SAVSBPM18-L-ABD(KC); 3 μl for SAVSBPM18 Affi-gel]; S, loaded sample; FT, flow-through fraction; W, Wash fractions; E, concentrated, pooled elution fraction. The volume for each fraction (S, FT, W1, W2 and E) is 1 ml. Volume applied to the gel: 4 μl for S, FT and W; 1 μl for E. Solid and empty arrowheads mark the positions for BSA and SAVSBPM18-L-ABD(KC), respectively. Arrow indicates the position of SAVSBPM18.