Figure 8. ²⁸Si particle irradiation causes increased levels of TET3 protein in the hippocampus.

Representative images show that radiation exposure causes increased TET3 levels in (a,b) DG and (c,d) CA1 regions of the brain and that is reduced by 5-ITU treatment (TET1, green; DAPI nuclear counterstain, blue). Deconvolution of images and quantification of TET3 demonstrate that irradiation induces increased protein levels at (e) 2 hrs, (f) 24 hrs and (g) 1 month post-irradiation and that 5-ITU treatment prior to irradiation protects against this increase. (h) Post-irradiation 5-ITU treatment also mitigates the radiation induced increase in TET3. Data are presented as mean ± SEM (N = 3–4 mice/group). P values are derived from ANOVA and Bonferroni’s multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001 for 0Gy, 0Gy + 5-ITU and 20cGy + 5-ITU as compared to 20cGy. ⁺P < 0.02 for 0Gy as compared to 0Gy + 5-ITU 0Gy + 5-ITU and for 0Gy as compared to 20cGy + 5-ITU. (a–d) Scale bars 20 μm.